Evaluation of A Rat Model of Parkinsons Disease by Injection of 6-OHDA into The Substantia Nigra

摘要：

A rat model of Parkinsons disease was established by direct injection of 6-OHDA into the substantia nigra unilaterally. Animals were assessed by D-amphetamine rotation test, anti-TH staining and detection of dopamine and its metabolites by HPLC-ECD. Rotational behavior experiment shown that the achievement ratio of PD model is 48.9％. Anti-TH staining result indicated that 6-OHDA treatment caused extensive loss of TH immunoreactivity in the substantia nigra and their projections to the striatum. Moreover, DA and its metabolites reduced remarkably in the substantia nigra and striatum of 6-OHDA lesioned rat model compared to control. Therefore, by evaluation of experimental animals, unilateral 6-OHDA induced substantia nigra degeneration can establish dependable rat model of Parkinsons disease with higher achievement ratio.

作者: Jian Mao Wei Yang Ran Wang YuJing Luan Hong Qing YuLin Deng

作者单位: School of Life Science and Technology, Beijing Institute of Technology, 100081 Beijing China

会议类型: 国际会议

会议名称: 2007 IEEE/ICME International Conference on Complex Medical Engineering-CME2007(CME2007 第二届国际复合医学工程学术大会)

会议地点: 北京

会议语种:英文

页码: 1438-1441

在线出版日期: 2007-05-23（万方平台首次上网日期，不代表论文的发表时间）