Studies on Kinetics of Reaction between Peroxynitrite and Tryptophan

摘要：

Peroxynitrite (ONOO-) is a potent cytotoxin, and it can damage tryptophan, tyrosine and other biomolecules. The kinetics of tryptophan damaged by peroxynitrite was studied by stopped-flow spectrophotometer at 25 ℃. When peroxynitrite is present in excess, there are many products formed including nitrotryptophan, hydroxytryptohan, N-formylkinurenine or dihydroxytryptophan, oxindole, and hydropyrroloindole. The kinetics of tryptophan loss can represent the kinetics of peroxynitrite damaging tryptophan as a whole. The kinetics of this reaciotn was studied by monitoring the decrease of fluorescence of tryptophan, and the apparrent second order rate, 2.85 ? 104 M-1s-1, and the apparent activation energy, 39.4 kJ mol-1 are derived.

作者: Wei Zhang Yunjing Luo Yan Zhen Peng Wei Rugang Zhong

作者单位: College of Life Science and Bioengineering, Beijing University of Technology, Beijing 100022, China College of Environmental and Energy Engineering, Beijing University of Technology, Beijing 100022, C

会议类型: 国际会议

会议名称: 2007 IEEE/ICME International Conference on Complex Medical Engineering-CME2007(CME2007 第二届国际复合医学工程学术大会)

会议地点: 北京

会议语种:英文

页码: 1630-1633

在线出版日期: 2007-05-23（万方平台首次上网日期，不代表论文的发表时间）