Assay for Affinity of Proteins onto Model Biomembrane
In this paper, immobilized artificial membrane (IAM) was selected as the model biomembrane, and packed into capillary column as stationary phase of liquid chromatography to screen the proteins having strong interaction with biomembrane rapidly. The affinity between IAM and ten common proteins (pepsin, bovine serum albumin, casein, transferring, myosin, insulin, hemoglobin, cytochrome C, trypsin, lysozyme), whose pI ranged from 2.0～11.5, were analyzed in different pH mobile phase including acidic, neutral and alkaline buffer. The optimized condition was obtained for the affinity of IAM and proteins. The proteins having strong affinity with IAM were screened out under each pH mobile phase. It can provide the experimental foundation for proteins to be immobilized onto biomembrane to study the membrane related interaction, separation, protein bioactivity, and drug screening and so on.
Jing Jiang Shanshan Wang Peixiang Ma Feng Qu Aiqin Luo Yulin Deng
School of Life Science and Technology, Beijing Institute of Technology, Beijing 100081, China
国际会议
北京
英文
1704-1708
2007-05-23（万方平台首次上网日期，不代表论文的发表时间）