HPCE Analysis of The Effect of Sargassum Fusiforme Polysaccharides on The Complex Mobility of Erythrocytes in Tumor-bearing Organisms
This study was to analyze the effect of Sargassum fusiforme polysaccharides (SFPS) on the electrophoretic mobility of erythrocytes in tumor-bearing organisms. A tumor mouse model was developed, and the mice were treated with high, medium, and low dosages of SFPS through abdominal injection for 7 d. Erythrocytes were collected and prepared into suspensions, and the complex motilities of the cells were measured using high performance capillary electrophoresis (HPCE). Experimental conditions: capillaries: 75 μm × 50 cm;buffer for electrophoresis: phosphate solution containing HPMC (0.1 mol/L, pH 7.4); injection pressure: 3.448 kPa; injection time: 10 s; separation voltage: 20 kV; column temperature: 25℃. The electroosmotic mobility was found to be 2.16×10-4 cm2·V-1·s-1, so SFPS can decrease the migration time of erythrocytes in tumor-bearing organisms, which was 17.56 for the control group, 13.96 for the low-dosage group, 12.9 for the medium-dosage group, 13.51 for the high-dosage group, while that for the normal group was 11.51; and increase its complex mobility, which was 1.06×10-4 cm2·V-1·s-1 for the control group, 1.19×10-4 cm2·V-1·s-1 for the low dosage group, 1.29×10-4 cm2·V-1·s-1 for the middle dosage group, 1.23×10-4 cm2·V-1·s-1 for the high dosage group, while that for the normal group was 1.45×10-4 cm2·V-1·s-1. The erythrocyte mobility for all three dosage groups differ significantly from that for the negative control (P＜0.01). SFPS can change the complex mobility of erythrocytes from tumor-bearing organisms toward the level for erythrocytes from normal organisms, and this may be because it can change the density of the surface charge on erythrocytes. So HPCE can be used as auxiliary tool for determining the physiological state and functions of erythrocytes.
high performance capillary electrophoresis (HPCE) Sargassum fusiforme erythrocyte complex mobility
JI Yu-bin JI Chen-feng WANG Chong
Center of Research in Life Sciences and Environmental Sciences, Harbin University of Commerce, Harbin 150076 China;Postdoctoral Program of the Institute of Materia Medica , Harbin University of Commerce, Harbin 150076 China
国际会议
北京
英文
1709-1712
2007-05-23（万方平台首次上网日期，不代表论文的发表时间）