会议专题

Quantification of Human Serum Albumin by Highly Sensitive Chemiluminescence Method

An efficient and highly sensitive chemiluminescence (CL) technique has been developed to detect human serum albumin (HSA). We have previously reported that chemiluminescence produced during interaction between FCLA-1O2 could be modified by the addition of HSA. By carefully selecting the condition of the reaction, the CL intensity could show a linear relationship to the HSA concentration over a clinically interested range of 5×10-9~8×10-8mol L-1 ,with a detection limit of 17.32ng/ml. The method is low cost and has a higher sensitivity than that of fluorescence spectra analysis, synchrony fluorescence scanning technique, and Rayleigh light scattering technique. The technique was used to test HSA in human urine obtained clinically, and the results were consistent to that from other conventional methods. The mechanism of the technique is discussed.

chemiluminescence (CL) Human Serum Albumin (HSA) FCLA singlet oxygen

Wei Xu Yanchun Wei Da Xing Qun Chen Shiming Luo

MOE Key Laboratory of Laser Life Science & Institute of Laser Life Science, SouthChina Normal University, Guangzhou 510631, China

国际会议

2007 IEEE/ICME International Conference on Complex Medical Engineering-CME2007(CME2007 第二届国际复合医学工程学术大会)

北京

英文

1722-1725

2007-05-23(万方平台首次上网日期,不代表论文的发表时间)