Quantification of Human Serum Albumin by Highly Sensitive Chemiluminescence Method
An efficient and highly sensitive chemiluminescence (CL) technique has been developed to detect human serum albumin (HSA). We have previously reported that chemiluminescence produced during interaction between FCLA-1O2 could be modified by the addition of HSA. By carefully selecting the condition of the reaction, the CL intensity could show a linear relationship to the HSA concentration over a clinically interested range of 5×10-9～8×10-8mol L-1 ,with a detection limit of 17.32ng/ml. The method is low cost and has a higher sensitivity than that of fluorescence spectra analysis, synchrony fluorescence scanning technique, and Rayleigh light scattering technique. The technique was used to test HSA in human urine obtained clinically, and the results were consistent to that from other conventional methods. The mechanism of the technique is discussed.
chemiluminescence (CL) Human Serum Albumin (HSA) FCLA singlet oxygen
Wei Xu Yanchun Wei Da Xing Qun Chen Shiming Luo
MOE Key Laboratory of Laser Life Science & Institute of Laser Life Science, SouthChina Normal University, Guangzhou 510631, China
国际会议
北京
英文
1722-1725
2007-05-23（万方平台首次上网日期，不代表论文的发表时间）