The Optimization of Proteins Assay under Extreme Acidic Condition with Capillary Electrophoresis
Capillary electrophoresis (CE) was an advanced method for protein separation. Under extreme acidic condition(pH＜3), fused silica capillary inner wall was protonated, so that the electrostatic adsorption between protein and inner wall of capillary could be suppressed effectively, which caused the resolution and column efficiency were improved. In this paper, four model proteins were separated under extreme acidic condition, and the separation rules were investigated by optimizing conditions, such as changing pH value and ion-strength of running buffer, adding high molecular polymer methylcellulose (MC) into running buffer, adding organic solvent methanol into running buffer.
Qian Li Feng Qu Hongmei, Gong Yulin Deng
School of Life Science & Technology, Beijing Institute of Technology P.O. Box 327 Beijing 100081, China
国际会议
北京
英文
1726-1729
2007-05-23（万方平台首次上网日期，不代表论文的发表时间）