Extraction Immunocompetent Sections of Agaricus Blazei Murill Polysaccharides by Membranes Technology
The bioactive of polysaccharides have relationship with their molecular weight. Agarcus blazei Murill (ABM) polysaccharides were extracted by membranes technology, and were graded according to the molecular weight using the combined method of microfiltration (MF), ultrafiltration (UF) and nanofiltration (NF) membrane modules. The effect of gradation were indicated by the high performance gel permeation chromatography (HPGPC), and lymphocyte proliferations in the different molecular weight ABM at the concentration range of 10～800μg/ml were detected with MTT method. The results of the experiment indicated that the MF-UF-NF combined method was effective for ABM, and were remarkably shown to increase lymphocyte proliferation at 100000Da of 100, 200, 400, 800μg/ml ABM (p＜0.001). The proliferation ratio was 258.7％～451.5％.
Ye xiao Liu hongmei Yan hong Zhong rugang
Beijing University of Technology, Beijing, 100022, China;Biochemical Engineering College of Beijing Biochemical Engineering College of Beijing Union University, Beijing, 100023, China Beijing University of Technology, Beijing, 100022, China
国际会议
北京
英文
1762-1765
2007-05-23（万方平台首次上网日期，不代表论文的发表时间）