A Novel Method to Enhance Sensitivity in HPLC Detection
The peak compression effect induced by velocity difference has been theoretically and experimentally established. L-tryptophan and 2-nitrophenol were used as model compounds. The peak compression of L-tryptophan and 2-nitrophenol was obtained by the difference of its migration velocity along the column induced by the adjustment of composition of mobile phase. A compression of analytes peak and reduced tailing was observed. Under optimum compression condition, 17-fold and 5.9-fold improvement in the detection sensitivity was obtained compared to elution under non-compressed conditions for L-tryptophan and 2-nitrophenol, respectively.
sensitivity HPLC detection
Yuxia Wei Shengyuan Xiao Xiaoling Mu Yong Zhu Gaofei Hu Yulin Deng Xinlin,Yang
School of Life Science&Technology, Beijing Institute of Technology (100081)
国际会议
北京
英文
1801-1805
2007-05-23（万方平台首次上网日期，不代表论文的发表时间）