Immobilization of Trypsin onto Artificial Membrane for the Possible Application in The Digestion Reactor of Proteins
This paper described a novel method to immobilize trypsin on IAM. The immobilization procedures involved the following steps: IAM was packed into the fused silica capillary (400μm I.D.×690μm O.D.×5cm length); subsequently, trypsin was dynamically immobilized on IAM by injecting 1mg/ml trypsin aqueous solution using the frontal chromatography. The activity of trypsin-micro-IMERs was on-line measured using BAEE (N-benzoyl-L-arginine ethyl ester) as the substrate of trysin. The results showed that the quantity of product BA and BAEE concentration increased linearly. The results illustrated that the quantity of BA at flow rate of 3 ul/min was obviously larger than that at flow rate of 6 ul/min. The bioactivity of immobilized trypsin was at least 69.3％ in 24h.
dynamica limmobilization trypsin-micro-IMERs BAEE
Yueying Liu RongJi Dai Feng Qu Weiwei Meng Yulin Deng
School of Life Science and Technology, Beijing Institute of Technology 100081
国际会议
北京
英文
1813-1817
2007-05-23（万方平台首次上网日期，不代表论文的发表时间）