Investigation on Insulin Tyrosine Modification Mediated by Peroxynitrite
Peroxynitrite (ONOO-), the product of superoxide (O2-·) and nitric oxide (NO·), has been implicated in diabetes and diabetic cardiovascular complications. Insulin, a mediator of these pathological processes, has an important role to maintain human blood glucose levels, and may be a potential target during accumulating ONOO- is formed in β-cells. ONOO- can inhibit the biological activity of insulin and affect its receptor binding capacities by modifying protein tyrosine residues. Herein, we analyzed the dose-dependent insulin tyrosine modification indicated by spectral changes. Also, the nitrated sites of insulin were identified by HPLC-MS analysis. The results show that an infusion of ONOO- caused a maximum observed yield of 2.7 3-nitrotyrosine per insulin subunit and led to different nitrated species. Moreover, Try-B26 appeared to be the most susceptible residue concerning with ONOO- mediated nitration.
Yunhai Wang Yunjing Luo Rugang Zhong
College of Life Science and Bioengineering, Beijing University of Technology, Beijing 100022, P. R. China
国际会议
北京
英文
1844-1847
2007-05-23(万方平台首次上网日期,不代表论文的发表时间)