Seperation and Determination of Glycyrrhizic Acid and Liquiritin in Licorice Using SPE-RP-HPLC
Glycyrrhizic acid (GA) and liquiritin are the two classes of bioactive components in licorice with known pharmacological effects. In our stud, different methods were compared to separate GA an- d liquiritin. Solid-phase extraction (SPE) method was verified to separate GA and liquiritin effectively. The method is fast, easy, reliable, and inexpensive; especially in the quality control. Moreover, we built a new method to determine Glycyrrhizic acid and liquiritin simultaneously using reversed phase high performance liquid chromatography (RP-HPLC) In the HPLC analyze, gradient elution and two wavelength were used . The regression line for GA was y =15340.6x+12.81, the regression for liquiritin line was y =9269.1x+20.63, The recovery rates were 101.3％ and 99.4％；RSD were 1.01％ and 0.92％.
Glycyrrhizic acid Liquiritin Solid-phase extraction RP-HPLC Separation
YU Yu-hong CAI Jian-jun DAI Rong-ji Deng Yu-lin YANG Kun MENG Wei-wei
School of Life Science and Technology, Beijing Institute of Technology, BeijingP. R. China 100081
国际会议
北京
英文
1887-1891
2007-05-23（万方平台首次上网日期，不代表论文的发表时间）