STS and RAPD Analyses Among Stylo Accessions and Identification of Distinct Lines for its Genetic Improvement
Stylo is an important range fodder legume of the tropical country (Ramesh et al., 1997; Chandra et al., 2006). In India this has been introduced largely from Australia and South America (Rai and Pathak, 1985). About forty species exist in genus Stylosanthes (Kirkbide and De Kirkbide, 1985) and of these five species namely, S. Scabra, S. Viscosa, S. Hamata, S. Guianensis and S. Seabrana are important and have been identified potential species in India. Both morphological and STS analyses (Liu and Musial, 1997) have indicated S. Viscosa and S. Seabrana as two diploid progenitors of tetraploid S. Scabra which is drought hardy and well suited for dry and low rainfall areas (Chandra et al., 2004). The recent introduction of S. Seabrana in this country has opened up new opportunities as it has been observed better suited to Indian condition for both green biomass and seed production (Chandra et al., 2006). Earlier studies have also indicated the use of hardy S. Scabra species in wasteland development (Pathak et al., 2004). S. Seabrana being one of the progenitors of the S. Scabra the nineteen lines of it was genetically characterized to delineate more closer lines that could be used in artificial synthesis of S. Scabra (Liu and Musial, 1997). Thirty two RAPD and 17 STS markers (Curtis et al., 1995; Liu et al., 1996; Manners et al., 1995; Reddy et al., 1996; Smith et al., 1995; Vander Stappen et al., 1999) have been used to assess the genetic relationships among the two progenitors and S. Scabra. In total 476 RAPD and 104 STS bands were scored. The PIC values of RAPD and STS markers ranged from 0.22 to 0.50 and 0.08 to 0.50 respectively. Two main clusters with both marker sets were observed when data were processed using Dice similarity coefficients and SHAN clustering in UPGMA module. Cluster one was further divided into two sub-clusters wherein sub-cluster-1 embodied all S. Seabrana and sub-cluster-2 possessed both accessions of S. Scabra. Second cluster contained both S. Viscosa lines. Bootstrap analysis also indicated strong clustering as many nodes has high bootstrap values (＞50). Results indicated more closeness of S. Seabrana (57％ with RAPD and 62％ with STS) to S. Scabra than S. Viscosa (53％ with RAPD and 60％ with STS) to S. Scabra. Accessions CPI-110372, IG-369 and EC-408405 made distinct cluster in sub-cluster-1 and observed close to S. Scabra. Therefore, these particular lines can be utilized in improvement of S. Scabra along with S. Viscosa.
Clustering Genetic relationships Polymorphic information content Polymorphism RAPD Similarity matrices STS Stylosanthes species.
S.Tewari A.Chandra
Crop Improvement Division, Indian Grassland and Fodder Research Institute, Jhansi, 284003, India
国际会议
The 2nd International Conference of Plant Molecular Breeding（第二届植物分子育种国际会议）
海南三亚
英文
88-89
2007-03-23（万方平台首次上网日期，不代表论文的发表时间）