DETECTION OF BIOMOLECULAR RECOGNITION BASED ON INTRINSIC MOLECULAR CHARGES
We have been investigating a new approach to detect biomolecular recognition without labeling materials. We have proposed the novel concept of a genetic field effect transistor (FET) which is based on the direct transduction of surface density change of charged biomolecules into electrical signal by the field effect. Potentiometric measurements of allele specific oligonucleotide hybridization, intercalation and primer extension at the surface of the gate insulator have been demonstrated using a genetic field effect transistor. Since DNA molecules are negatively charged in an aqueous solution, hybridization event at the gate surface leads to charge density change in the channel of the FET and can be transduced into electrical signal directly without any labeling for target DNA molecules. One of the unique features of our method is to utilize DNA binders such as intercalators as charged species for double stranded DNA after hybridization. because they are ionized and carry positive charges in an aqueous solution. Single-base mismatch of the target DNA could be successfully detected by primer extension reaction. The genetic FET platform is suitable for a simple, accurate and inexpensive system for SNP typing in clinical diagnostics.
Molecular charge field effect transistor SNPs analysis DNA sequencing
Yuji Miyahara Toshiya Sakata
Biomaterials Center, National Institute for Materials Science,1-1, Namiki, Tsukuba, Ibaraki, 305-004 Center for NanoBio Integration, The University of Tokyo,7-3-1 Hongo, Bunkyo-ku, Tokyo 113-8656, Japa
国际会议
The 4th International Forum on Post-genome Technologies(4IFPT)(第四届国际后基因组生命科学技术学术论坛)
杭州
英文
8-11
2006-09-25（万方平台首次上网日期，不代表论文的发表时间）