RNA TRAP BY ELECTRIC AND HYDRAULIC FORCE FIELDS IN MICROFLUIDIC CHANNEL

摘要：

On this research, we discovered a phenomenon that charged biomolecules such as DNA are trapped and accumulated at the constricted position of a microfluidic chip when both hydro pressure and electric field are applied in opposite directions. Compared to DNA, RNA is more difficult to be trapped because of its instability. By mild denaturation which changes RNA comformation from block-like structures into chain-like structures, we succeeded in trapptng RNA using this technique. This technique is expected to establish easy and practical device as a direct total RNA extraction tool from single celi or living tissues.

关键词： RNA extraction single cell analysis molecular trap

作者: Yuzuru Takamura Kunimitsu Ueno Wako Nagasaka Yuichi Tomizawa Yoshiteru Nakamori Eiich Tamiya

作者单位: JAPAN ADVANCED INSTITUTE OF SCIENCE AND TECHNOLOGY (JAIST),School of Materials Science and 1-1 Asahi JAPAN ADVANCED INSTITUTE OF SCIENCE AND TECHNOLOGY (JAIST),School of Knowledge Science 1-1 Asahidai, JAPAN ADVANCED INSTITUTE OF SCIENCE AND TECHNOLOGY (JAIST),School of Materials Science and 1-1 Asahi

会议类型: 国际会议

会议名称: The 4th International Forum on Post-genome Technologies(4IFPT)(第四届国际后基因组生命科学技术学术论坛)

会议地点: 杭州

会议语种:英文

页码: 37-38

在线出版日期: 2006-09-25（万方平台首次上网日期，不代表论文的发表时间）