SNP DETECTION IN ANGIOTENSINOGEN (ACT) GENE USING MAGNETIC NANOPARTICLES WITH DUAL-COLOR HYBRIDIZATION
A single nucleotide polymorphism (SNP) within the angiotensinogen (AGT) gene was detected by dual-color (Cy3 and Cy5) hybridization based on the the method using magnetic nanoparticles (MNPs). M235T is a common single base change which encodes threonine instead of methionine at position 235 in AGT gene and is a genetic marker for susceptibility to hypertension. A pair of short (13 bases) and allele specific probes were designed to detect SNP in AGT. Biotin-labeled PCR products were captured on 80 μg streptavidin coated MNPs and interrogated by hybridization with dual-color probes at 38 ℃ to determine SNP, and then genotype of each sample can be simultaneously identified by scanning the microarray printed with the denatured fluorescent probes. Homozygous samples had a fluorescence ratio over 10 (match: mismatch signal), whereas heterozygous samples had ratios near 1. 0. The results indicate that this method could be applied as a rapid, effective and high-throughput tool for SNP detection.
Angiotensinogen gene M235T polymorphism single nucleotide polymorphism magnetic nanoparticles
Song Li Hongna Liu Quanguo He Nongyue He
Chien-Shiung Wu Laboratory, Southeast University, Nanjing 210096, China;Key Laboratory of Green Pack Key Laboratory of Green Packaging and Application of Biological Nanotechnology of Hunan Province, Hu
国际会议
The 4th International Forum on Post-genome Technologies(4IFPT)(第四届国际后基因组生命科学技术学术论坛)
杭州
英文
41-45
2006-09-25（万方平台首次上网日期，不代表论文的发表时间）