NEW CONSIDERATION ON UITRA-LOW-COST SEQUENCING FOR THE HUMAN WHOLE GENOME
Recently, the exploration for ultra-low-cost and high-throughput DNA sequencing is being attracted more and more attentions, and tremendously developments have been being achieved The most important approaches are based on the array platforms on which genomic DNA (gDNA) fragments fixed arbitrarily on the solid substrates and formed as an random gDNA microarray. and high resolution images of fluorescence or chemical illumination were taken from the gDNA array during the nucleotide extension process according the gDNA templates. Basically, there are two routes to prepare gDNA array, which is amplified DNA fragment by PCR or single molecule with one copy DNA fragment. These two routes are difficult to perform the whole genome sequencing, because in the first route the PCR reaction could hardly amplify all the sequences from 3 billion human genome, and in the second route the single molecule sequencing is not reliable enough to get relative long length. In this presentation, we will introduce the new route to prepare the DNA templates with rolling cycle amplification (RCA) for whole genome, and the products of RCA are single DNA molecules with more than 1000 copies of the genomic DNA fragment. We have successfully to sequence the RCA products with modified Sanger Method, pyrosequencing protocol, and the hybridization identification. Our preliminary results showed that it is promising to realize the ultra-low-oost whole genomic sequencing.
Zuhong Lu
State Key Laboratory for Bioelectronics,Southeast University, Nanjing 210096, China
国际会议
The 4th International Forum on Post-genome Technologies(4IFPT)(第四届国际后基因组生命科学技术学术论坛)
杭州
英文
46-48
2006-09-25（万方平台首次上网日期，不代表论文的发表时间）