ASSESSMENT OF TaqMan-MGBPROBES USEFULNESS IN THE GENOTYPING OF GSTP1 EXON 5 SNP:COMPARISON TO THE PCR-RFLP METHOD

摘要：

A high-throughput genotyping method has been developed to detect single-nucleotide polymorphisms(SNP). This method utilizes the 5 -nuclease activity of Taq polymerase in conjunction with fluorogenic TaqMan-MGB Probes. GSTP1 exon 5 gene presents a single-nucleotide polymorphism (A to G) that results into an amino-acid substitution (Ile to Val). This polymorphism affects enzyme activity and widely studied in the context of SNP related to cancer susceptibility. The goal was to evaluate TaqMan-MGB Probes genotyping method usefulness in detecting a wellknown SNP in comparison to PCR-RFLP. In 321 unrelated individuals who were ethnic minority from Guizhout GSTP1 exon 5 SNP was analyzed by TaqMan-MGB probes genotyping method and PCR-RFLP method. The genotyping using both methods resulted in the characterization of 226 (70.4％) homozygous wild-type (AA), 92 (28. 7％) heterozygote (AG) and 3 (0.9％) homozygous mutant (GG). All samples were also identically genotyped by the two methods. Results show that TaqMan-MGB Probes genotyping method is a good alternative to classical PCR-RFLP method in genotyping SNPs. It is rapid, reproducible, and highly sensitive and could be applied toward fully automated large-scale genotyping

关键词： TaqMan-MGB Probes SNP GSTP1 PCR-RFLP

作者: Wei Zhang Xilin Ren Keren Shan Changxue Wu Yi Li Yan Xiao Yan Zhao Xiaolan Qi Yan He Zhizhong Guan

作者单位: The Key Lab of Molecular Biology, Guiyang Medical College, Guiyang 550004, China

会议类型: 国际会议

会议名称: The 4th International Forum on Post-genome Technologies(4IFPT)(第四届国际后基因组生命科学技术学术论坛)

会议地点: 杭州

会议语种:英文

页码: 81-85

在线出版日期: 2006-09-25（万方平台首次上网日期，不代表论文的发表时间）