ASSOCIATION ANALYSIS OF SINGLE NUCLEOTIDE POLYMORPHISMS IN THE OLR1 GENE WITH CORONARY HEART DISEASE BASED ON DNA MICROARRAY APPROACH
One of the fruits of the Human Genome Project (HGP) is the discovery of millions of DNA sequence variants in the human genome, especially the single nucleotide polymorphisms (SNPs). A dense set of SNP markers opens up the possibility of studying the genetic basis of complex diseases by population approaches. Coronary heart disease (CHD) is a progressive disorder caused by a complex interaction of both genetic and environmental factors. Its suggested that the lectin-like oxidized receptor 1 (OLR1) gene may play an important role in the development of CHD. Two SNPs of this gene were selected for genotyping with 238 CHD patients and 233 healthy controls of Chinese population. Our current approach was based on the aeryl-modified glass slides directly printed with PCR product solution to fabricate a sample microarray. Dual- color fluorescence hybridization was applied for useful and high-throughput genotyping. The current results showed that the frequency of genotypes for the two polymorphisms in the OLR1 gene had no significant difference between patients and controls, but the difference among groups of patients was significant, which suggested that the OLR1 gene had association with the risk of coronary heart disease in Chinese population.
CHD SNP DNA microarray OLR1 gene
Beili Sun Lu Cheng Pengfeng Xiao Zuhong Lu
State Key Laboratory of Bioelectronics, Southeast University, Nanjing 210096, China
国际会议
The 4th International Forum on Post-genome Technologies(4IFPT)(第四届国际后基因组生命科学技术学术论坛)
杭州
英文
86-90
2006-09-25（万方平台首次上网日期，不代表论文的发表时间）