DETECTION OF HYPERMETHYLATION OF p16ink4a BY ROLLING CIRCLE AMPLIFICATION
(0) Aberrant DNA methylation of the CpG site is among the earliest and most frequent alterations in cancer. Detection of promoter hypermethylation of cancerrelated genes may be useful for cancer diagnosis or the detection of recurrence. p16, an inhibitor of the cyctin D-dependent protein kinases, is a classical tumor suppressor gene, and its inactivation is closely associated with carcinogenesis. p16 hypermethylation could be detected in each stage, which is consistent with the finding that aberrant methylation of p16 is a very early event in carcinogenesis. We have developed an new procedure for detecting DNA methylation of the human pl6Ink4a gene. The procedure is based on the chemiluminescent detection with rolling cycle amplifyication DNA from human hepatocellular tumor cell lines and whole blood cells of healthy human. Methylation status of human p16Ink4a gene was detected and a good reproducibility was observed in several parallel experiments. Our experiments successfully demonstrated that the rolling circle amplification based chemiluminescent methodology could be applied as a highthroughput tool to determine hypermethylation status of the investigated genes.
Rolling circle amplification methylation p16 chemiluminescence
Junfeng Luo Zhixiang Wu Yan Wang Wenli Zheng Zuhong Lu
SState Key Laboratory of Bioelectronics, Southeast University, Nanjing 210096, China
国际会议
The 4th International Forum on Post-genome Technologies(4IFPT)(第四届国际后基因组生命科学技术学术论坛)
杭州
英文
107-110
2006-09-25（万方平台首次上网日期，不代表论文的发表时间）