MOLECULAR CLONING PROPHENOLOXIDASE FROM THE CHINESE MITTEN CRAB ERIOCHEIR SINENSIS AND ITS EXPRESSION IN TREMOR DISEASE
The Chinese mitten crab Eriocheir sinensis is an important valuable freshwater aquicultural crustacean in China. But in the last few years, the aquiculture of crab has been particularly badly hit by epidemics of a serious disease, tremor disease. The prophenoloxidase (proPO) is a key protein in the innate immune system in crustacean and stored in haemocyte granules. The enzyme can be released into the hemolymph against infectious pathogen. But so far no information was reported on cDNA sequence of proPO in the crab and the relation of proPO mRNA expression to the infectious diseases with Spiroplasma. In this study, we cloned 202 bp cDNA sequence of proPO isolated from haemocytes of E. sinensis by a reverse-transcription polymerase chain reaction (RT-PCR). By comparison with other decapods, the 202 bp nucleic acid sequence of E. sinensis proPO exhibited the highest similarity to that of Cancer magister (87％), Scylla serrata (85％), and Padfastacus leniuscutus (84％). In addition, the crabs were infected by Spiroplasma, considered to be a pathogen to induce tremor disease of crab. Haemocytes were obtained from the infected crabs at 12 h, 24 h, 48 h, 72 h, 5 d, 10 d and 15 d respectively with 5 crabs in each time point. The mRNA expression of proPO was analyzed by RT-PCR, The results showed that the low mRNA expression of proPO was detected in control crabs. The expression levels of proPO significantly changed with infectious course of Spiroplasma and symptom of tremor disease. This suggests that the proPO expression is related to the health status of crabs and the proPO system in E. sinensis may play an important role in non-self recognition and host immune reactions against Spiroplasma.
Prophenoloxidase Eriocheir tinensis nucleotide sequence mRNA expression
Dakai Mu Jianlin Pan Long Yi Wen Wang Yayi Hou
Immunology and Reproductive Biology Lab, Medical School and State Key Laboratory of Pharmaceutical B Freshwater Fishery Institute of Jiangsu Province, Nanjing 210017, China Jiangsu Key Laboratory for Bioresources Technology, College of Biological Sciences,Nanjing Normal Un
国际会议
The 4th International Forum on Post-genome Technologies(4IFPT)(第四届国际后基因组生命科学技术学术论坛)
杭州
英文
134-136
2006-09-25（万方平台首次上网日期，不代表论文的发表时间）