SEQUENCE ANALYSIS OF BRACHYVRY (T) GENE FOR THE SHORT-TAIL MUTATION IN MOUSE
A mutant mouse line was produced using N-ethyl-N-nitrosourea mutagenesis. Linkage analysis revealed that the causative gene is linked to D17Mitl43 on the chromosome 17. According to gene mapping information and morphological characteristics of the mutant mice, it was concluded that the Brachyury (T) gene is a strong candidate gene for the short-tail phenotype. In order to confirm above conclusion, a pregnant mutant heterozygous female mouse (B6 - St) mated with mutant heterozygous male mouse (B6 - St) was anatomized for obtaining embryos on the 8th-9th pregnant day, and a pregnant normal B6 female mouse mated with B6 male one was dose same thing. Then total RNA is extracted from the dissected fetus from the short-tail mouse embryos and the normal B6, and the T genes were amplified using RT-PCR respectively. The RT-PCR product was purified using FasrGene kit and sequenced. The sequencing results showed that a T to A substitution was found at nucleotide 67 (the 965292-th locus of the entire gene) on the 2nd exon of B6 - St T gene. The substitution led to a loss of 67 bp coded of the 2nd exon, and result in a loss of the protein function coded by the T, and eventually caused the short tail phenotype in mice. So far there has been no literature report about this mutated T gene, so the mutated gene reported in this work is a novol allele for the T gene. This allele may be named TSh.
Short-tail mouse Brachyury(T) gene RT-PCR sequence allelic gene
Yixiang Shao Bing Chen Chun Liu Zhengfeng Xue Huihua Mao Baojin Wu Houda Li
Laboratory Animal Center of Nantong University, Nantong 226001, China Comparative Medicine Center of Yangzhou University, Yangzhou 225009 , China
国际会议
The 4th International Forum on Post-genome Technologies(4IFPT)(第四届国际后基因组生命科学技术学术论坛)
杭州
英文
145-148
2006-09-25（万方平台首次上网日期，不代表论文的发表时间）