SEQUENCING TEMPLATE PREPARATION FOR PYROSEQUENCING BASED ON ROLLING CIRCLE AMPLIFICATION

Pyrosequencing is a new DNA sequencing technique developed in recently years, which has been developed to be arobust and potential DNA sequencing technique. Sequencing template for pyrosequencing is usually obtained using PCR. But, PCR conditions must be optimized and PCR requires heavy thermal cycling instrument. In this study we described a method to successfully prepare sequencing template for pyrosequencing based on rolling circle amplification.
Rolling circle amplification pyrosequencing template
Zhiqiang Pan Pengfeng Xiao Dongrui Zhou Yanqiang Li Zuhong Lu
State Key Laboratory of Bioelectronics, Southeast University,Nanjing 210096, China
国际会议
The 4th International Forum on Post-genome Technologies(4IFPT)(第四届国际后基因组生命科学技术学术论坛)
杭州
英文
149-153
2006-09-25(万方平台首次上网日期,不代表论文的发表时间)