SEQUENCING TEMPLATE PREPARATION FOR PYROSEQUENCING BASED ON ROLLING CIRCLE AMPLIFICATION

摘要：

Pyrosequencing is a new DNA sequencing technique developed in recently years, which has been developed to be arobust and potential DNA sequencing technique. Sequencing template for pyrosequencing is usually obtained using PCR. But, PCR conditions must be optimized and PCR requires heavy thermal cycling instrument. In this study we described a method to successfully prepare sequencing template for pyrosequencing based on rolling circle amplification.

关键词： Rolling circle amplification pyrosequencing template

作者: Zhiqiang Pan Pengfeng Xiao Dongrui Zhou Yanqiang Li Zuhong Lu

作者单位: State Key Laboratory of Bioelectronics, Southeast University,Nanjing 210096, China

会议类型: 国际会议

会议名称: The 4th International Forum on Post-genome Technologies(4IFPT)(第四届国际后基因组生命科学技术学术论坛)

会议地点: 杭州

会议语种:英文

页码: 149-153

在线出版日期: 2006-09-25（万方平台首次上网日期，不代表论文的发表时间）