SOLID-PHASE AMPLIFICATION ON MAGNETIC NANOPARTICLES FOR DETECTION OF SINGLE NUCLEOTIDE POLYMORPHISM
Single nucleotide polymorphisms (SNPs) are most common form of genetic variation in the human genome. SNPs promise great medical utility, since they give rise to individual gene variants that can alter susceptibility to common diseases and predict disease progression as well as individual responses to drugs. To meet these goals, a precise, cost-effective, high-throughput method for determination of hundreds of thousands of genotypes performed in many individuals is required. We have developed a novel approach for genotyping of single nucleotide polymorphisms (SNPs) based on PCR amplification on magnetic nanoparticles. Using one primer bounds to the surface of magnetic nanoparticles (MNPs), DNA was then amplified and covalently immobilized on the MNPs in parallel, then interrogated by hybridization with a pair of dual-color fluorescent (Cy3, Cy5) probes to determine SNP. The methylenetetrahydrofolate reductase (MTHFR) gene C677T polymorphism of 9 different samples was detected and their fluorescent signals of 9 samples were quantified. Without any purification and concentration of PCR products, application of this strategy to large-scale studies will reduce the time and procedure of genotyping a vast number of SNPs.
Magnetic nanoparticlesm solid-phase PCR single nucleotide polymorphism detection
Hongna Liu Song Li Zhifei Wang Nongyue He
Key Laboratory of Green Packaging and Application of Biological Nanotechnology of Hunan Province,Hun Chien-Shiung Wu Laboratory, Southeast University, Nanjing 210096, China
国际会议
The 4th International Forum on Post-genome Technologies(4IFPT)(第四届国际后基因组生命科学技术学术论坛)
杭州
英文
159-162
2006-09-25（万方平台首次上网日期，不代表论文的发表时间）