THE ANALYSIS OF GENE EXPRESSION PROFILING FOR PHARMACODYNAMIC ACTION OF COMPOSITION OR COMBINATION OF JASMINOIDIN AND CHOLALIC ACID AFTER FOCAL CEREBRAL ISCHEMIA IN MICE
To elucidate the gene expression of focal cerebral ischemia mice responses to herbs treatment, we analyzed the expression of 372 mouse genes in focal cerebral ischemia before and after treatment with Jasminoidin and Cholalic acid given alone or in combination. We used generally accepted methods, including TTC staining, behavior test, as well as micro and ultra microscopy which can dynamically and accurately monitor pathological and physiological changes after cerebral ischemia on earlier period. Based on changes in gene expression, we identified 57 genes that accurately discriminated among the three treatments. Discriminating genes included those involved in apoptosis, signal transduction, cell cycle control and transporter activity. There are some differences in gene expression between Jasminoidin and Cholalic acid given alone and in combination, which is not simple accumulation of profiling of gene expression of single drug. These data indicate that gene expression of Jasminoidin and Cholalic acid after focal cerebral ischemia in mice share different pathways of genomic response to the same composition treatment, that changes in gene expression are treatment-specific and that gene expression can illuminate differences in biological response to drug combinations versus single agents.
Jasminoidin cholalic acid microarray focal cerebral ischemia
Zhanjun Zhang Pengtao Li Wensheng Zhang Yongyan Wang
The Key Laboratory of Traditional Chinese Medicine Protection and Utilization,Beijing Normal Univers Beijing University of Traditional Chinese Medicine, Beijing 100029 ,China
国际会议
The 4th International Forum on Post-genome Technologies(4IFPT)(第四届国际后基因组生命科学技术学术论坛)
杭州
英文
168-170
2006-09-25（万方平台首次上网日期，不代表论文的发表时间）