XPD Lys751Gln GENETIC POLYMORPHISM AND LUNG CANCER RISK IN A CHINESE POPULATION
To investigate the XPD Lys751Gln genetic polymorphism related to lung cancer susceptibility in a Chinese population, a 1 : 1 matched case-control study was performed in which 200 hospital controls were matched to the 200 original lung cancer cases. The Lys751Gln polymorphism of XPD gene was analyzed by di-allele-specific-amplification with artificially modified primer (diASA-AMP) method. To validate the accuracy of genotype scoring by diASAAMP method, 115 samples were genotyped using the traditional polymerase chain reaction and restriction fragment length polymorphism (PCR-RFLP) analysis. The results show that a significant association between the XPD Lys751Gln mutation and lung cancer risk, seen by comparing the Lys/Gln+Gln/Gln with Lys/Lys genotype (OR = 3. 32, 95％CI=1. 85-5. 96). After stratifying according the smoking status, no significant gene-smoking interaction was observed. Results of genotyping using diASA-AMP and PCR-RFLP showed a consistency rate of 96. 5％ and a stronger correlativity and consistency by the X2 test (Kappa=0. 90, 95％CI=0. 81 - 1. 00). This study suggests that XPD Lys751Gln genetic polymorphism might play an important role in susceptibility of lung cancer in the Chinese population. The diASA-AMP method was accurate and reliable and could serve as a rapid detection of the gene polymorphisms related to tumor susceptibility in a population.
Lung cancer genetic polymorphism XPD diASA-AMP
Geyu Liang Yuepu Pu Lihong Yin
School of Public Health, Southeast University, Nanjing 210009, China
国际会议
The 4th International Forum on Post-genome Technologies(4IFPT)(第四届国际后基因组生命科学技术学术论坛)
杭州
英文
183-186
2006-09-25（万方平台首次上网日期，不代表论文的发表时间）