A METHOD FOR DETECTION OF SEQUENCE SPECIFIC DNA-BINDING PROTEIN

摘要：

Transcription factors played central role in drug development and disease diagonosis. There was a need for robust high throughput methods to analysis of transcription factors and sequence-specific DNA. Here unimolecular hairpin oligonucleotide probes were immobilized on polyacrylamide gel pad arrays and subsequently converted to double strand DNA arrays for detection of transcription factors. RhNF-kB p50 homodimer was detected by the miroarrays. The results indicated the method a economical, sensitive and efficient method and could be applied to analysis such as interaction between protein and sequence specific DNA as well as single nucteotide polymorphism site recognition.

关键词： Transcription factors unimolecular hairpin oligonucleotide DNA polyacrylamide gel pads rhNF-kB p50 homodimer

作者: Qin Pan Yunfei Bai Dongrui Zhou Jinke Wang Zuhong Lu

作者单位: State Key Laboratory of Bioelectronics,Southeast University, Nanjing 210096, China

会议类型: 国际会议

会议名称: The 4th International Forum on Post-genome Technologies(4IFPT)(第四届国际后基因组生命科学技术学术论坛)

会议地点: 杭州

会议语种:英文

页码: 188-191

在线出版日期: 2006-09-25（万方平台首次上网日期，不代表论文的发表时间）