ASSOCIATION BETWEEN GENE EXPRESSION OF METABOLIZING ENZYMES AND ESOPHAGEAL SQUAMOUS CELL CARCINOMAS IN CHINA
To determine whether the basal expression level of genes involved in metabolism process to carcinogens is associated with the risk of ESCC, a case-control study was performed and the relative expression levels of four metabolism genes (CYP2E1, GSTP1, MTHFR and NQO1) were determined with peripheral blood mononuclear cells (PBMCs). 100 patients with newly diagnosed, untreated ESCC and 117 healthy controls matched with cases on age, sex, nation and residence were recruited in the case-control study. Expression levels of four genes were measured by quantitative real-time reverse transcription-PCR detected by SYBR Green I with ft-actin as an internal control for blood samples. Compared with normal controls, the relative expression levels of four genes, including CYP2E1, GSTP1, MTHFR and NQO1, were significantly altered in ESCC patients (P=0. 036, P=0.0001, P=0.009, P =0. 002, respectively). Analyzed with the mean of relative expression level in the controls as the cut-off point, the result exhibited that the increased risk for ESCC were significantly associated with reduced expression of GSTP1 (OR=3.644, 95％CI: 1.947-6.823) and NQOl (OR= 1.870, 95％CI: 1.046-3.345). When adjusted with age, sex, smoking status and alcohol use the increased risk for ESCC were significantly associated with reduced expression of GSTP1, MTHFR and NQOl. And GSTPl mRNA showed the steady associated with the risk for ESCC (OR=2. 640) in the model of stepwise regression analysis. Reduced expression of GSTPl in PBMCs was significantly associated with the risk for ESCC (OR = 2. 640), suggesting an important etiology clue to descending GSTPl expression for the early progression of ESCC in Huaian population of China.
Gene expression quantitative real time PCR esophageal squamous cell carcinomas phase Ⅰ and Ⅱmetabolism gene
Ran Liu Libong Yin Yuepu Pu
School of Public Health, Southeast University, Nanjing 210009,China
国际会议
The 4th International Forum on Post-genome Technologies(4IFPT)(第四届国际后基因组生命科学技术学术论坛)
杭州
英文
197-200
2006-09-25（万方平台首次上网日期，不代表论文的发表时间）