CG13142, A NOVEL PROTEIN, CAN PARTIALLY RESCUE Dmp53 OVEREXPRESSION PHENOTYPE

摘要：

p53, a key tumor suppressor, plays magnificent roles on cell-cycle arrest, DNA repair, cellular senescence, differentiation and apoptosis. Overexpression of Necdin in N1H3T3 and SAOS2 cell lines can block p53 - mediated apoptosis while does not reduce p53 - induced cell growth arrest. In this paper, we cloned a novel gene encoding CG13142 protein, which can interact with Drosophifa MAGE homologue DmMAGE, and can partially rescue phenotype of Dmp53 when they co-expressed in eye discs under the control of pGMR-Ga14 in vivo. Expression level of CG13142 increased in Dmp53 - deficient flies. Furthermore, CG13142 was found directly interact with Dmp53 via its C-termtnal by GST pulldown. These results indicate that CG13142 can partially rescue Dmp53 Overexpression phenotype in some cell context through direct interaction, and its expression level may be regulated by Dmp53.

关键词： Dmp53 DmMAGE CG13142 Overexpression

作者: Chengqi Lin Zhuojuan Luo Hongyan Ren Wei Xie

作者单位: Key Laboratory of Developmental Genes and Human Diseases, Ministry of Education Southeast University, Nanjing 210009, China

会议类型: 国际会议

会议名称: The 4th International Forum on Post-genome Technologies(4IFPT)(第四届国际后基因组生命科学技术学术论坛)

会议地点: 杭州

会议语种:英文

页码: 201-205

在线出版日期: 2006-09-25（万方平台首次上网日期，不代表论文的发表时间）