DEVELOPMENT OF COMPETITIVE IMMUNOASSAYS FOR MORPHINE DETECTION ON A PROTEIN CHIP
Competitive immunoassays on a protein chip as a detective tool for morphine were developed. An antibody chip based on the competition-restrain reaction principle between immobilized antimorphine and fluorescein-labeled BSAmorphine complex was developed with confocal scanner analysis method to detect the interaction of antibody and antigen of morphine. The optimal concentrations of antimorphine and BSA-marphine-Cy3 are 0. 031 3 mg/ml and 0. 012 5 mg/ml, respectively; the linearity range is 0. 01- 10 ng/ml. The limit of detection is 0. 001 ng/ml. The cross-reactions of antimorphine with methyl Benzedrine, amphetamine were not detected. But a certain extent cross-reaction between antimorphine and codeine was detected. The recovery of morphine and its metabolized products in tested urine is between 91. 2％ and 109. 2％, with a detected limit of 0. 02 ng/ml. The result suggested that the antibody chip is a sensitive, specific, easy-operating, time-saving, high-throughput and sample/reagent-minimized method, and is useful in forensic medicine detection, effect monitoring during the process of abstaining from drug and drug-screening.
Forensic science morphine protein chip competitive immunoassay
Yang Cao Lingang Gu Wusheng Zhao Zuhong Lu
State key laboratory of Bioelectronics, Southeast University, Nanjing 219006, China;Division of the Division of the Criminal Investigation Department Jiangsu Provincial Public Security Bureau State key laboratory of Bioelectronics, Southeast University, Nanjing 219006, China
国际会议
The 4th International Forum on Post-genome Technologies(4IFPT)(第四届国际后基因组生命科学技术学术论坛)
杭州
英文
206-210
2006-09-25（万方平台首次上网日期，不代表论文的发表时间）