A NOVEL LIVING CELL MANIPULATION TECHNOLOGY USING NANONEEDLE AND AFM

摘要：

In this report, we introduce a novel living cell manipulation technology using an atomic force microscopy (AFM) and a nanoneedle. The nanoneedle was prepared etched and sharpened into diameter of 200 nm using focused ion beam. Three dimensional operation and insertion of the nanoneedle can be monitored in real time using AFM We succeeded in a high efficient gene transfer into primary cultured human mesenchymal stem cell (hMSCs) using DNA-adsorbed nanoneedle.

关键词： Cell manipulation nanoneedle AFM gene transfer

作者: Chikashi Nakamura SungWoong Han Ikuo Obataya Yosuke Imai Teruyuki Nagamune Jun Miyake

作者单位: Research Institute for Cell Engineering, National Institute of Advanced Industrial Science and Techn Research Institute for Cell Engineering, National Institute of Advanced Industrial Science and Techn Research Institute for Cell Engineering, National Institute of Advanced Industrial Science and Techn Department of Biotechnology and Life Science, Tokyo University of Agriculture and Technology,Koganei Department of Chemistry and Biotechnology, The University of Tokyo, Bunkyo-ku, 113-8654, Japan Research Institute for Cell Engineering, National Institute of Advanced Industrial Science and Techn

会议类型: 国际会议

会议名称: The 4th International Forum on Post-genome Technologies(4IFPT)(第四届国际后基因组生命科学技术学术论坛)

会议地点: 杭州

会议语种:英文

页码: 231-232

在线出版日期: 2006-09-25（万方平台首次上网日期，不代表论文的发表时间）