AMPLIFICATION OF DNA DETECTION SIGNAL USING GOLD NANOPARTICLES AS AMPLIFIER BY QUARTZ CRYSTAL MICROBALANCE

摘要：

Gold nanoparticles were utilized for amplifying the frequency signal of DNA detection using quartz crystal microbalance (QCM), DNA Immobilization and hybridization were monitored by QCM frequency shifts. Streptavidin-nanogold conjugations were connected with target DNA to amplify the frequency signal. It suggests that the frequency shift increases with the decrease of nanogold diameter and the increase of streptavidin-nanogold concentration. The optimal ratio of streptavidin-nanogold stock solution: PBS was 7 μ1:50 μ1. The sensitivity of this enhancement detection is 100 fM The complementary and mismatched target DNA could be distinguished clearly.

关键词： Gold nanoparticle DNA quartz crystal microbalance

作者: Yun Yang Libo Nie Song Li Quanguo He Hong Chen

作者单位: Hunan Key Laboratory of Green-Packaging and Application of Biological Nanotechnology,Hunan Universit Hunan Key Laboratory of Green-Packaging and Application of Biological Nanotechnology,Hunan Universit Department of Life Science and Technology, Central South Forestry University, Changsha 410004, China

会议类型: 国际会议

会议名称: The 4th International Forum on Post-genome Technologies(4IFPT)(第四届国际后基因组生命科学技术学术论坛)

会议地点: 杭州

会议语种:英文

页码: 233-236

在线出版日期: 2006-09-25（万方平台首次上网日期，不代表论文的发表时间）