ELECTROSPUN POLYMER NANOFIBERS PACKED MICRO-COLUMN FOR THE SOLID-PHASE EXTRACTION OF PAPAVERINE IN HUMAN PLASMA
A novel microextraction procedure based on micro-column packed with polystyrene electrospun nanofibers as the sorbents was presented as a simple, rapid and effective sample pretreatment technique coupled with high-performance liquid chromatography (HPLC-UV) for analysis of papaverine in human plasma. In the extraction procedure, the plasma sample was loaded onto the micro-column, and pushed through the column bed by a gas tight plastic syringe. The targets were absorbed onto the nanofibers. After washing sorbent with water, the analyte was eluted with a small aliquot of methanol, which can be directly injected onto the assay system. Various factors were considered systematically. Parameters such as fiber diameter, packing amount of the naofibers, and pH of liquid phase were evaluated. The procedure was validated in terms of linearity (y=0. 999 4 for concentrations ranging from 10 to 5 000 ng/mL), precision (4. 4 and 1. 6％, respectively at 100 and 500 ng/mL), sensitivity (5 ng/mL for LOD) and extraction recovery (80. 6％-90. 2％). The developed procedure was found suitable for forensic investigations and was considered a good alternative to the liquid-liquid extraction methods normally used for the determination of this compound in biological media.
Solid-phase extraction preconcentration electrospun nanofiber plasma papaverine LC-UV
Xuejun Kang Hongmei Wei Yiyun Zhang Liqin Chen Zhongze Gu
State Key Laboratory of Molecular and Biomolecular Electronics, Southeast University,Nanjing 210096, State Key Laboratory of Molecular and Biomolecular Electronics, Southeast University,Nanjing 210096, School of Public Health, Southeast University,Nanjing 210009, China
国际会议
The 4th International Forum on Post-genome Technologies(4IFPT)(第四届国际后基因组生命科学技术学术论坛)
杭州
英文
241-245
2006-09-25（万方平台首次上网日期，不代表论文的发表时间）