EXPERIMENTS AND MODELING ON LMPEDANCE-BASED BIOSENSORS FOR CELL ATTACHMENT AND CYTOTOXICITY RESEARCH
Instruments for the study of living cells have historically been of significant importance for such things as basic neuroscience and cell biology. Comparing with existed measurement techniques; microelectrode arrays provide not only a much simpler measurement, but also a label-free and noninvasive detection. More recent work has extended the use of Cell-Based Biosensors (CBB) to pharmaceutical screening, environmental monitoring, and toxin detectioa During these works, the change of impedance can be easily figured out in experiment, however, why impedance varied is still not well developed in the cell-based biosensors theory, since cellular interaction and adhesion on electrode surfaces is a dynamic and complicated process. In this work, we use a real-time cell electronic sensing (RT-CES? system as cell-based assays. We investigated the impedance variation caused by cell attachment, cell proliferation, and cytotoxicity treatment on celt Then a new equivalent circuit model based on the experimental results was developed to explain those changes. This model involves both biological and physical meanings. In improvement, a more universal modeling could be achieved from our initial modeling. Further experimental results perfectly matched and supported our modeling and assumptions. We also qualified some detailed parameters, which offer a convenient and quantitative means of assessing the kinetics of cell adhesion. Utilizing the results generated from modeling and following experiments by SEM, TIRFM and Confocal Microscopy, we found that there could be a misunderstanding in judging distance between the basal cell surface and substratum in some earlier literature reports. Last, we treated cancer cells with several kinds of widely used drugs. The consequent response of cells accord well with our modeling suggests. Finally, the practicality of using microelectrode based sensor technologies for monitoring the behavior of cultured cells in different treatments is discussed and concluded.
Cell-based biosensor cell attachment proliferation cytotoxicity modeling
Zaozao Chen Xiaobo Wang Mengdie Qian Xuan Liu Ying Luan Zuhong Lu
Chien-Shiung Wu Laboratory, Department of Biological Science and Medical Engineering,Southeast Unive ACEA Biosciences, Inc. , 11585 Sorrento Valley Rd. Suite 103, San Diego, CA 92121, USA
国际会议
The 4th International Forum on Post-genome Technologies(4IFPT)(第四届国际后基因组生命科学技术学术论坛)
杭州
英文
246-251
2006-09-25（万方平台首次上网日期，不代表论文的发表时间）