GEL-BASED DNA MICROARRAY FOR HIGH-THROUGHPUT SNP GENOTYPING BY UNIVERSAL DUAL-COLOR FLUORESCENT DETECTOR
Robust, inexpensive, high-throughput methodologies are neededfor the analysis of molecular markers. This study describes a practical approach that allows high-throughput genotyping of single nucleotide polymorphisms (SNPs) and mutations by a pair of universal dual-color fluorescent detectors hybridizing on gel-immobilization arrays. The method relies on the polyacrylamide gel-based DNA microarray platform by copolymerization acrylamide-modified PCR products with acrylamide monomers, in which N. N, N′, N′ - tetramethylethylenediamine (TEMED) is introduced after the prepolymer being spotted on the acryl-modified slides. The array was hybridized with a pair of specific probes and the two universal dual-color fluorescent detectors. Our experiment successfully demonstrate that the gel-based microarray method with the universal dual-color hybridization could be applied as a simple, robust method for SNP genotyping, especially as low-cost and high-throughput tool for many different SNPs analysis in a large number of individuals.
SNP DNA microarray gel-immobilization hybridization
Lu Cheng Beili Sun Pengfeng Xiao Yuanzhuo Zheng Yu Sun Yao Yang Zuhong Lu
State Key Laboratory of Bioelectronics, Southeast University, Nanjing 210096, China
国际会议
The 4th International Forum on Post-genome Technologies(4IFPT)(第四届国际后基因组生命科学技术学术论坛)
杭州
英文
252-256
2006-09-25（万方平台首次上网日期，不代表论文的发表时间）