STUDY OF ATP BIOLUMINESCENCE REACTION
ATP (adenosine triphosphate) bioluminescence technology is based on the reaction between ATP and firefly luciferin-luciferase which generates measurable fluorescence. In this paper, a study on the fluorescence generated from the ATP bioluminescence reaction and the influence of other factors was primarily investigated The experimental results show that the fluorescence has an emission wavelength of about 550 nm from its emission spectrum tested by a spectrophotometer in the wavelength scan mode. It results from the oxidation decarboxylation of luciferin with the catalysis of luciferase. The intensity of the fluorescence decays with the reaction time, which may be alleviated by introducing an ATP regeneration system to realize the regeneration of ATP in samples. In addition, the ATP concentration also affects the intensity of the fluorescence when the firefly luciferin is excessive. Its experimentally observed that the fluorescence intensity correlates well with the ATP concentration in the range of 0. 5 - 5 μmol/L, which is accordant with the Michaelis-Menten equation. However, in order to put the ATP bioluminescence technology into practice more widely in whole food industry, further research work will be done in the future for exploiting some portable apparatus and corresponding sensors which are inexpensive, sensitive and suitable to detect samples in situ.
ATP bioluminescence fluorescence firefly luciferin-luctferase ATP concentration
Jinping Luo Weiwei Yue Baoshan He Aiyu Zhou Xinxia Cai
State Key Lab of Transducer Technology, Institute of Electronics, Chinese Academy of Sciences,Beijing 100080, China;Graduate School of Chinese Academy of Sciences, Beijing 100080, China
国际会议
The 4th International Forum on Post-genome Technologies(4IFPT)(第四届国际后基因组生命科学技术学术论坛)
杭州
英文
277-279
2006-09-25（万方平台首次上网日期，不代表论文的发表时间）