APPLICATION OF OPTICAL WAVEGUIDE LIGHTMODE SPECTROSCOPY TO DETECT THE NFκB P50
The binding activity of NFκB p50 to the DNA duplex with specific binding site was studied using optical waveguide lightmode spectroscopy (OWLS). The universal probe was immobilized online onto the amino silanized sensor of OWLS by glutaraldehyde covalent coupling. The oligonucleotide with the binding site of NFκB p50 homodimer was mixed with NFκB p50 homodimer. The mixture was pre-incubated and injected into the OWLS system and then the hybridization process of the DNA-protein complex with the immobilized universal probes in the surface of waveguide sensor was detected by the OWLS. In our experiment, the different concentrations of p50 homodimer in detection samples were tested with the OWLS technique. As low as 16. 7 ng/ml p50 homodimer in the samples was detected with OWLS technique. As a high sensitivity label-free detection tool, the OWLS system can be extensively used in biomedical research targeted at the interaction of DNA with DNA-binding proteins.
OWLS label-free detection NFκB p50 DNA duplex
Zhongwei Wu Quanjun Liu Xiao Xie Lingwei Wu Tian Wen Ying Zhuang Yunfei Bai Zuhong Lu
State key laboratory of Bioelectronics, Southeast University, Nanjing 219006, China
国际会议
The 4th International Forum on Post-genome Technologies(4IFPT)(第四届国际后基因组生命科学技术学术论坛)
杭州
英文
299-302
2006-09-25（万方平台首次上网日期，不代表论文的发表时间）