DERIVATISATION OF SOLID SUPPORTS FOR PRESYNTHESIZED OLIGONUCLEOTIDE MICROARRAYS
This report emphasizes the surface chemistry strategies that are required to ensure immobilization of presynthesizedamino-modified otigonucleotide probe onto the surface of glass in microarray format. We here report various derivatisations for highly efficient activation of glass slides. Our work is based on the initial modification of glass with primary amino groups using aminopropyltriethoxysilane and in a following step, the application of homobifunctional linkers, such as glutaraldehyde or 1, 4-phenylenediisothiocyanate (PDITC) and prefabricated dendritic macromolecules with 48 primary amino groups in its outer sphere which could subsequently be activated and crosslinked with a homobifunctional linker. The resulting DNA microarrays derivatised simultaneously with different methods were studied by the comparison of relative areal densities of immobilized probes using the fluorescence scanner. The results indicate that the dendrimer-activated surfaces display a surface coverage with better spot homogeneity than that with conventional microarrays containing linear linkers. However, 4-mtrophenyl-chloroformate activated surfaces have excellent morphology and resistance against repeated thermal washing.
Microarray probe immobilization PAMAM
Tian Wen Quanjun Liu Zuhong Lu
State Key Laboratory of Bioelectronics, Southeast University, Nanjing 210096, China
国际会议
The 4th International Forum on Post-genome Technologies(4IFPT)(第四届国际后基因组生命科学技术学术论坛)
杭州
英文
306-309
2006-09-25（万方平台首次上网日期，不代表论文的发表时间）