DNA SEQUENCING WITH ON-CHIP SANGER METHOD
DNA sequencing is the central technology in our understanding of biology. In the post-genome time, a cheaper,faster, or more sensitive technology for DNA sequencing is required. And DNA microarrays are widely applied in life science, including sequencing technology for its high throughout, large scale and parallel. We applied chain-terminating inhibitors on DNA microarrays in order to develop an ideal sequencing method. This method is based on multiple-base extension on DNA microarrays. Two branches of our study, the single-step method and the multiplestep method, were operated respectively. The single-step method was designed to validate the feasibility of the onchip Sanger technology, and we successfully sequenced 4 bases by this method. The multiple-step method is a real fulfillment of on-chip Sanger technology. In the experiment of multiple-step method, the results of the first four steps were consistent with what we anticipated, but the result of the fifth step did not accord with the exception because of the incompleteness extension of a previous step. Some future works need to be done to solve this problem and to involve this method into a practical sequencing method. The combing of single molecule detection technology in the future will make this on-chip Sanger method a more superior sequencing approach than the existing method.
Sequencing DNA microarray sanger sequencing ddNTP
Jing Tu Yunfei Bai Zuhong Lu
State Key Laboratory of Bioelectronics, Southeast University,Nanjing 210096, China
国际会议
The 4th International Forum on Post-genome Technologies(4IFPT)(第四届国际后基因组生命科学技术学术论坛)
杭州
英文
314-318
2006-09-25（万方平台首次上网日期，不代表论文的发表时间）