MICROARRAY-IN-A-TUBE PCR ASSAY FOR HIV-1 DRUG RESISTANT MUTATION DETECTION

摘要：

A novel microarray assay for the HIV drug resistance mutation detection was reported. In this assay, the microarray was arranged on the inner surface of a specially designed* optically transparent, plastic Eppendorf cap. The full analysis/inspection processes, including multi-(RT)-PCR, hybridization, and fluorescence detection, were carried out inside the encapsulated Eppendorf tube. The Microarray-in-a-tube assay PCR assay was successfully applied for detecting a number of HIV - 1 drug resistance mutations of HIV - 1 reverse transcriptase gene including K65R, K103N. V106A, V108I, Y181C, Y184M and Y188C. The assay system is low cost and simple to use, and prevents the false results associated with contamination of the PCR products.

关键词： Microarray-in-a-tube HIV drug resistant mutation

作者: Quanjun Liu Ying Zhuang Zhongwei Wu Tian Wen Xie Xiao Wei Zhao Wei Liu Zuhong Lu

作者单位: Chien-Shiung Wu Laboratory, Southeast University, Nanjing 210096, China The Affiliated Second Nanjing Hospital of the Medical College, Southeast University,Nanjing 210003,

会议类型: 国际会议

会议名称: The 4th International Forum on Post-genome Technologies(4IFPT)(第四届国际后基因组生命科学技术学术论坛)

会议地点: 杭州

会议语种:英文

页码: 334-337

在线出版日期: 2006-09-25（万方平台首次上网日期，不代表论文的发表时间）