NOVEL ENZYME IMMUNOASSAY BASED ON POTENTIOMETRIC MEASUREMENT OF MOLECULAR ADSORPTION EVENTS BY EXTENDED-GATE FIELD-EFFECT TRANSISTOR SENSOR
We propose here a novel enzyme immunoassay based on a potentiometric measurement of molecular adsorption events by an extended-gate field-effect transistor (FET) sensor. Adsorption of thiol compounds such as alkanethiol on a gold electrode of the FET sensor resulted in a larger potential change of about 700 mV. Moreover, it was found that the amount of thiol compounds depended on its rate of adsorption on the gold surface. To construct the enzyme immunoassay system using the FET sensor, the enzyme chemistry of acetylcholinesterase (AChE) to generate thiol compounds was used and combined with the enzyme-linked immunosorbent assays. After the AChE-catalyzed reaction, the amount of the antigen was obtained by detecting the adsorption rate of the generated thiol compound on the gold surface by the FET sensor. It was also clarified that the measurement stability was improved with the high frequency voltage of 10 kHz or more superimposed onto the reference electrode. The signal corresponding to concentration (1-250 pg/mL) of the Interleukin 1β was obtained by the FET sensor with the voltage of 1 MHz superimposed onto the reference electrode. Using this enzyme immunoassay, the FET sensor can successfully detect Interleukin 1β of 1 pg/mL.
FET sensor extended-gate imiminosensor adsorption frequency
Masao Kamahori Yu Ishige Maki Shimoda
Central Research Laboratory, Hitachi, Ltd. , 1-280 Higashi-Koigakubo, Kokubunji- shi,Tokyo 185-8601, Hitachi ULSI Systems Co. , Ltd .1-280 Higashi-Koigakubo, Kokubunji-shi, Tokyo 185-8601, Japan
国际会议
The 4th International Forum on Post-genome Technologies(4IFPT)(第四届国际后基因组生命科学技术学术论坛)
杭州
英文
338-341
2006-09-25（万方平台首次上网日期，不代表论文的发表时间）