SURFACE FUNCTIONALIZATION WITH DENDRIMER MOLECULES FOR OPTICAL WAVEGUIDE LIGHTMODE SPECTROSCOPY

摘要：

We developed a new surface modification method, which uses a hydrophilic dendrimer, SuperfectTM originally a transfection reagent, to modify the sensor waveguide for label-free detection of Optical Waveguide Lightmode Spectroscopy (OWLS). The modified 3-dimensional surface can absorb the antibody through electrostatic force. Here we used the insulin-anti-insulin antibody (IAA) as an example of peptide-protein interaction to investigate the parameters of this method. 2 βIU/ml (approximately 71 pg/ml) insulin in the solution can be detected by the Superfect-IAA surface using direct Ag-Ab binding protocol, monitoring with OWLS technique. It was proved that the antigen can be detected sensitively and quantitatively in real-time. It is also easy to regenerate the sensor chip without losing sensing performance, compared with the chemical modifications.

关键词： Dendrimer surface modification integrated optics optical waveguide lightmode spectroscopy immunoassay

作者: Xiao Xie Quanjun Liu Zhongwei Wu Lingwei Wu Zuhong Lu

作者单位: State Laboratory of Bioelectroinics, Southeast University, Nanjing 210096, China

会议类型: 国际会议

会议名称: The 4th International Forum on Post-genome Technologies(4IFPT)(第四届国际后基因组生命科学技术学术论坛)

会议地点: 杭州

会议语种:英文

页码: 342-345

在线出版日期: 2006-09-25（万方平台首次上网日期，不代表论文的发表时间）