THE OPTIMAL CONCENTRATION OF FLUORESCENCE PROBE FOR REAL-TIME PCR ON DAN MICROARRAY
There are many kinds of methods used for quantitative PCR experiment. However, these methods can simultaneity detect 7 kinds of different gene fragments at best. Considering Microarray as a high throughput method could be detected many more gene site simultaneous. The Real-time PCR on DNA microarray developed in our laboratory. In this paper, we adopt the Gene Frames as the container of the PCR reaction on the microscope slides. During the PCR reaction, the fluorescence intensity of special fluorescence probe and non-special fluorescence probe (Mark) which were immobilized on the glutaraldehyde derived glass slide were be real-time detected by the scanner, and the change of the intensity of the special probe showed that the method is available. Through the experiment the optimal of the concentration of the probes was 5 μM. This result will be very useful for the further study of the Real-time PCR on DNA microarray.
Real-time PCR microarray fluorescence probe
Ying Zhuang Lingwei Wu Zhongwei Wu Xiao Xie Tian Wen Quanjun Liu
State Key Laboratory of Bioelectronics, Department of Biological Science and Medical Engineering.Southeast University, Nanjing 210096, China
国际会议
The 4th International Forum on Post-genome Technologies(4IFPT)(第四届国际后基因组生命科学技术学术论坛)
杭州
英文
346-349
2006-09-25（万方平台首次上网日期，不代表论文的发表时间）