EXPRESSION OF ALPHAB-CRYSTALLIN GENE IN HEPATOCELLULAR CARCINOMA AND ITS PROGNOSTIC SIGNIFICANCE
In recent years, gene chips, bioinformatics has provided useful help by in-depth scientific research. This article, difference expression gene AlphaB-Crystatlin (CRYAB) were selected by gene chips and bioinformatics from rat liver cancer models. And RT-PCR and Immunohistochemistry were used to confirm CRYAB expression in human HCC at mRNA and protein level. At the same time, Kaplan-Meier survival analysis and Cox proportion risk models were used for 98 cases of HCC patients undergoing clinical analysis. Results showed that CRYAB (p=0. 041), Tumor diameter (P=0. 001), Venous invasion (P=0. 003). Lymphatic invasion (P=0. 029) statistically significant, as the impact HCC prognosis factors. COX multi-factor analysis: CRYAB (P=0. 048), Venous invasion (P =0.008) is the independent HCC prognosis factors. And the experiments also showed that using bioinformatics and gene chips, could help us find a new gene or new possible diagnostic marker.
Hepatocellular carcinoma gene chip CRYAB immunohistochemistiy prognosis
Qi Tang Yuefang Liu Xiaojing Zhu Huilin Zhang Jin Zhu Zhenqing Feng Jianping Zhang Xiaohong Guan
Department of Pathology, NJMU, Nanjing 210029, China Key Laboratory of Antibody technique of Ministry of health, Nanjing 210029, China Department of General Surgery, the Second Affiliated Hospital of NJMU, Nanjing 210011, China
国际会议
The 4th International Forum on Post-genome Technologies(4IFPT)(第四届国际后基因组生命科学技术学术论坛)
杭州
英文
361-364
2006-09-25（万方平台首次上网日期，不代表论文的发表时间）