IDENTIFY AND SCREEN THE ASSOCIATED GENES AND POTENTIAL DIAGNOSITC MARKERS OF RAT PRIMARY LIVER CANCER
In order to study the difference of gene expression in Rats primary liver cancer (T) and normal tissue of Hver(N), and to screen for associated gene and diagnostic protein markers in early stage of primary liver cancer ( PLC) by oligonucleotide microarrays and bioinformatics, rat primary liver cancer were induced by DEN. and Affymetrix Rat Genenome 230. 2. 0. GeneChip was used to detect the gene expression profile difference in T and N tissue. Bioinformatics was undertaken to analyze the test results and screen diagnostic markers. As compared with normal liver tissue, a total 447 genes (EST/Full length) in primary liver cancers were found to be different with more than two fold in expression levels. Of the 447 genes, 271 were up-reguiated (Log ratio≥2), and 176 were down-regulated (Log Ratio≤-2). According to the Gene Ontology (GO) classification and Excel overlapping analysis, these genes were classified into differential biological process (204) and molecular function (178), 12 potential diagnostics protein markers have been screened out. In conclusion, tumor associated gene and potential diagnostics markers in early stage of primary liver cancer was successfully identified and screened by a combination of boinformatics and Affymetrix microarry technology.
Primary liver cancer gene chip gene expression profile diagnostics marker
Kaikun Liu Yuefang Liu Jin Zhu Xiaojing Zhu Huilin Zhang Qi Tang Zhenqing Feng Jianping Zhang
Department of General Surgery, the Second Affiliated Hospital of NJMU, Nanjing 210011, China Department of Pathology, NJMU, Nanjing 210029, China Department of Pathology, NJMU, Nanjing 210029, China;Huadong Medical Institute of Biotechniques, Nan
国际会议
The 4th International Forum on Post-genome Technologies(4IFPT)(第四届国际后基因组生命科学技术学术论坛)
杭州
英文
370-374
2006-09-25（万方平台首次上网日期，不代表论文的发表时间）