17β-ESTRADIOL SUPPRESSES THE CYTOTOXICITY AND VIABILITY OF MURINE SPLENIC NK1.1+ Cells in vitro
Uterine natural killer (uNK) and 17β-estradiol (E2) play a key role in implantation and pregnancy and their number or level change with the stage of pregnancy. But the effect of sex hormones on NK cell activity is not still clarified. In the present study, using a magnetic cell sorter (MACS), highly purified and viability NK cells were obtained from C57BL/6J mice spleen. After treatment of various concentrations (10 nM- 1μM) of 17β- estradiol for 24 h, the results showed that NK cell cytotoxicity was decreased. Furthermore, the proiiferative viability, phenotypic molecules including activation-associated markers (CD69, CD122) and inhibitory receptors (CD94, Ly49), as well as two critical cytokines IFN - γ and TNF - α were detected by flow-cytometry. Our data demonstrat that E2 - induced inhibition of NK cytotoxicity is manifested at multiple levels, including decrease NK cell numbers, increase expression of inhibitory receptor (CD94) and blockade of NK cell cytokine secretion (IFN - γ) which necessary for NK differentiation and maturation. Our results suggest that the inhibitory effects of E2 on NK cell activity may be related to tolerance of maternal body to fetus during the pregnancy.
17β-estradiol (E2) natural killer cell proiiferative viability cytotoxicity
Sha Hao Junli Zhao Yali Hu Yayi Hou
Immunology and Reproductive Biology Lab, Medical School and State Key Laboratory of Pharmaceutical B The Affiliated Drum Tower Hospital of Nanjing University Medical School, Nanjing 210008, China
国际会议
The 4th International Forum on Post-genome Technologies(4IFPT)(第四届国际后基因组生命科学技术学术论坛)
杭州
英文
408-411
2006-09-25（万方平台首次上网日期，不代表论文的发表时间）