ALTERATION OF ppGALNAcTs GENE EXPRESSION IN THE 1, 25-DIHYDROXYVITAMIN D3 INDUCED DIFFERENTIATION OF HUMAN ACUTE MONOCYTIC LEUKEMIA CELL LINE SHI-1
To elucidate the role of ppGalNAcTs in the differentiation of SHI-1, we use 1,25 -(OH)2D3 as a inducer. In the present study, we have demonstrated that 1,25 -(OH)2D3 induced acute monocytic leukemia cell line SHIIdifferentiation into normal mature monocytes as confirmed by morphologic studies and analyzing CD11b expression on the cell surface. The real-time PCR results show that ppGalNAc-T1 increased after the cells were exposed to 1. 25 -(OH)2D3. The expression of ppGalNAc - T2 and - T3 was unaffected while ppGalNAc - T4 decreased We suggest that ppGalNAc - T1 and - T4 gene expression may be the molecular indicator in the differentiation of acute monocytic leukemia.
ppGalNAcTs 1 25(OH)2D3 differentiation SHI-1 cells
Xianghong Guo Saiyu Hang Liyan Zhu Shiliang Wu
Department of Biochemistry and Molecular Biology, Institute of Biochemistry Engineering,Suzhou University, Suzhou 215123, China
国际会议
The 4th International Forum on Post-genome Technologies(4IFPT)(第四届国际后基因组生命科学技术学术论坛)
杭州
英文
432-435
2006-09-25（万方平台首次上网日期，不代表论文的发表时间）