APPLICATION OF REAL-TIME CELL ELECTRONIC SENSING (RT-CES) TECHNOLOGY TO DYNAMIC MONITORING OF DRUG INTERACTION WITH TARGET CELLS
The uses of cell-based assays are crucial for understanding the efficacy, specificity and mechanism of drug interaction with target cells. Using the conventional end point assays can only detect very specific cellular changes such as viability, occurring after certain durations of drug exposure, which provides no dynamic information with respect to living cells. Here, we have applied a real-time cell electronic sensing (RT-CES? system, which uses the circleon-line microelectrode array for label-free, real time, automated dynamic detection. The RT-CE system offers an innovative solution to a number of limitations with current systems. In the experiment, we treated cancer cells with several kinds of widely used antineoplastic drugs including antimetabolite, alkylating agents, antibiotic, annmicrotubule, platinum compounds and new drugs to assess drug potency. We found that A549 cells were sensitive to 5-FU, Ara-C, ACTD, BLM, EADM, DM, VLB, VCR, TAXOL, CBP, DDP and Staurosprine, and resisitent to MTX. To conclude, the RT-CES system can be applied to a wide variety of cell-based assays in both basic academic research and pharmaceutical screening.
Cell detecting antineoplastic drugs the RT-CES system real time
Xuan Liu Zaozao Chen Mengdie Qian Ying Luan Xiaobo Wang Zuhong Lu
Chien-Shiung Wu Laboratory, Southeast University, Nanjing 210096, China
国际会议
The 4th International Forum on Post-genome Technologies(4IFPT)(第四届国际后基因组生命科学技术学术论坛)
杭州
英文
440-442
2006-09-25（万方平台首次上网日期，不代表论文的发表时间）