CONSTRUCTION OF EXPRESSION VECTOR OF siRNA SPECIFIC FOR ALPHA-FETOPROTEIN AND IDENTIFICATION OF ITS EFFICIENCY IN FU97 GASTRIC CARCINOMA CELL LINE
Many studies have examined the biological activities of Alpha-fetoprotein (AFP); however, the modulatory role of AFP in neoplastic growth remains unclear. Three hairpin siRNA expressing plasmids (pSIREN -DNR-DsRedExpress Donor - Vector - AFP - siRNA1/2/3) were constructed and transfected into FU97 (AFP - producing gastric adenocarcinoma cell line). At 48 h after transfection, the silencing efficiency to AFP gene expression was analyzed by RT - PCR and immunoassays. The influence to FU97 on its growth and apoptosis after silencing the AFP gene were observed by MTT and flow cytometry. The results of RT - PCR and immunoassays showed that the plasmids could inhibit AFP mRNA and protein expression at 48 h after transfection. The pSIRFJN - DNR - DsRed - Express Donor Vector - AFP - siRNAl was more effective than other two. These plasmids could obviously inhibit FU97 cell growth and promote FU97 cell apoptosis.
Alpha-fetoprotein RNA interfering FU97 gastric carcinoma gene silencing
Anla Hu Wanshan M Xiaoli Ma Yanfei Jia Fang Zhou Yunshan Wang
College of Public Hygiene, Anhui Medical University, Hefei 230032, China;Central Laboratory, Jinan C Qianfushan Hospital, Jinan 250014, China Central Laboratory, Jinan Central Hospital, Clinical Medical College,Shandong University, Jinan 2500
国际会议
The 4th International Forum on Post-genome Technologies(4IFPT)(第四届国际后基因组生命科学技术学术论坛)
杭州
英文
461-464
2006-09-25（万方平台首次上网日期，不代表论文的发表时间）