DYNAMIC EXPRESSION AND ALTERATION CHARACTERISTIC OF INSULIN- LIKE GROWTH FACTOR-Ⅱ DURING THE DEVELOPMENT OF HEPATOCELLULAR CARCINOMA
Insulin-like-growth factor Ⅱ (IGF -Ⅱ ) is a mitogenic polypeptide having structural similarity to proinsulin and widely distributed in various fetal and neonatal human and rat tissues, including liver. It has also been implicated in the pathogenesis of neoplasm of different tissues, including liver of rats and mea IGF - Ⅱ is believed to exert their effect during cellular proliferation through endocrine, autocrine and paracrine mechanisms. In recent years, the reactivation of IGF - Ⅱ gene has been reported in variety of neoplasms including HCC in rats. The overexpression of IGF-Ⅱ has been found during the development of HCC but the mechanism hasnt been known yet. Therefore, we made the rat hepatoma models induced with 2-fiuorenylacetamide (2-FAA) to explore the pathological mechanism of the dynamic expression and alteration of IGF - Ⅱ during development of HCC. The morphological changes of rat livers were observed by pathological examinations (HE staining). The dynamic changes of IGF - Ⅱ in rat livers and sera were quantitatively analyzed by enzyme linked immunosorbent assay (ELISA). Simultaneously, the expression and cellular distribution of liver IGF - Ⅱ were detected by immunohistochemistry. Our results confirmed that the hepatic IGF - Ⅱ may participate in hepatocyte canceration and detection of IGF - Ⅱ expression during HCC development could be a useful molecular marker for early diagnosis and prognosis of HCC.
Rat hepatoma IGF-Ⅱ immunohistochemistry ELISA dynamic expression
Liwei Qiu Dengfu Yao Lei Zong Wei Wu Xinhua Wu
Research Center of Clinical Molecular Biology, Affiliated Hospital of Nantong University,Nantong 226001, China
国际会议
The 4th International Forum on Post-genome Technologies(4IFPT)(第四届国际后基因组生命科学技术学术论坛)
杭州
英文
469-471
2006-09-25（万方平台首次上网日期，不代表论文的发表时间）