EPITOPE EXAMINATION FOR ANTIBODY PREPARATION AND EXPRESSION ANALYSIS OF HUMAN POLYPEPTIDE: N-ACETYGALACTOSAMINYLTRANSFERASE-2 PROTEIN
O-glycosylation of proteins is initiated by a family of UDP-N-acetylgalactosamineipolypeptide N-acetylgalactosaminyltransferases (Ga1NAc-T). To study subcellular localization of ppGa1NAcT2, the secondary structure and surface properties of ppGa1NAcT2 was studied and we found that the prevailing epitopes of ppGa1NAcT2 existed in the RICIN domain, and we developed polyclonal antibodies against ricin of ppGa1NAcT2. These antibodies titer were 1:16000 detected by ELISA and they had good reactivity and high speciality in Western blot. We have localized ppGa1NAcT2 to Golgi apparatus by immunofluorescence. The results suggested that ppGa1NAc - T2 might take part in modifying glycoproteins in the Golgi complex.
Polypeptide N-Acetygalactosaminyltransferase-2 epitope domain antibody
Saiyu Hang Hejuan Hu Liyan Zhu Shi Yan Yan Wu Shiliang Wu
Department of Biochemistry and Molecular Biology, Suzhou University, Suzhou 215007, China Department of Biochemistry and Molecular Biology, Suzhou University, Suzhou 215007, China;Institute
国际会议
The 4th International Forum on Post-genome Technologies(4IFPT)(第四届国际后基因组生命科学技术学术论坛)
杭州
英文
480-482
2006-09-25（万方平台首次上网日期，不代表论文的发表时间）